We are providing a brief summary of the lectures presented live during the training course. For the hands-on sessions, all materials are provided in the respective web pages.

The following topics were covered:

  • Nucleic acid extractions
  • Measures of success
  • Approaching a new sample
  • Chordates
  • Plants (Phenol:Chloroform-based methods)
  • Plants (nuclei isolation + salting out)
  • Filamentous fungi high molecular weight (HMW) DNA extractions
  • Insect HMW DNA extractions
  • Ultra-high molecular weight (UHMW) DNA extraction
  • Ultra-long DNA sequencing kit protocol (SQK-ULK114)
  • Handling & storing UHMW DNA
  • Sequencing output
  • HMW & UHMW DNA extractions – What’s next?