Syllabus
Day 1
- Course overview and introduction to nanopore sequencing
- Considerations for high molecular weight DNA extraction and library preparation
- Principles and practice of modified base detection
- Hands on: Basecalling, sequence alignment, and quality control
- Hands on: Base modification detection
- Hands on: Visualisation of base modification data - part I
Day 2
- Hands on: Manipulation of base modification data
- Case study: Using nanopore sequencing to study DNA replication dynamics
- Hands on: Alternative method for single molecule base modification detection
- Hands on: Visualisation of base modification data - part II
- Review modification detection pipeline and Q & A